Review



anti human il 12p70  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    R&D Systems anti human il 12p70
    Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, <t>α-IL-12p70</t> Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).
    Anti Human Il 12p70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human il 12p70/product/R&D Systems
    Average 93 stars, based on 67 article reviews
    anti human il 12p70 - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "IL-12 drives the expression of the inhibitory receptor NKG2A on human tumor-reactive CD8 T cells"

    Article Title: IL-12 drives the expression of the inhibitory receptor NKG2A on human tumor-reactive CD8 T cells

    Journal: Nature Communications

    doi: 10.1038/s41467-024-54420-w

    Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, α-IL-12p70 Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).
    Figure Legend Snippet: Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, α-IL-12p70 Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).

    Techniques Used: Expressing, Control, Inhibition



    Similar Products

    anti human il 12p70  (R&D Systems)
    93
    R&D Systems anti human il 12p70
    Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, <t>α-IL-12p70</t> Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).
    Anti Human Il 12p70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human il 12p70/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    anti human il 12p70 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    human il-12 p70 antibody  (Bio-Techne corporation)
    94
    Bio-Techne corporation human il-12 p70 antibody
    Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, <t>α-IL-12p70</t> Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).
    Human Il 12 P70 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human il-12 p70 antibody/product/Bio-Techne corporation
    Average 94 stars, based on 1 article reviews
    human il-12 p70 antibody - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    cd40l blocking  (R&D Systems)
    93
    R&D Systems cd40l blocking
    Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, <t>α-IL-12p70</t> Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).
    Cd40l Blocking, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd40l blocking/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    cd40l blocking - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    anti human il12p70  (R&D Systems)
    93
    R&D Systems anti human il12p70
    Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, <t>α-IL-12p70</t> Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).
    Anti Human Il12p70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human il12p70/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    anti human il12p70 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    il 12p70  (R&D Systems)
    93
    R&D Systems il 12p70
    a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail <t>of</t> <t>IL-12,</t> IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .
    Il 12p70, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 12p70/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    il 12p70 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    mab219  (R&D Systems)
    93
    R&D Systems mab219
    a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail <t>of</t> <t>IL-12,</t> IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .
    Mab219, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mab219/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    mab219 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, α-IL-12p70 Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).

    Journal: Nature Communications

    Article Title: IL-12 drives the expression of the inhibitory receptor NKG2A on human tumor-reactive CD8 T cells

    doi: 10.1038/s41467-024-54420-w

    Figure Lengend Snippet: Sorted naïve CD8 T cells were activated with CD3/CD28 beads for 24 hours, rested for 2 days and restimulated with CD3/CD28 beads for 8 days in the presence of the indicated cytokines with or without TGF-β. a Data from a representative donor and ( b ) summary of NKG2A expression after 8 days of culture are shown ( n = 5). c Expression of NKG2A by CD8 T cells after stimulation of PBMC with SEB in the presence of isotype control, α-IL-12p70 Ab, α-CD40 Ab or both. Data from one representative donor are shown. d Summary of ( c ) (left) and percentage inhibition of NKG2A expression by CD8 T cells in the different culture conditions (right) ( n = 4). e Detection of IL-12p70 in the culture supernatants of PBMCs stimulated for 3 days with SEB in presence or not of α-CD40 Ab (left) and percentage inhibition of IL-12p70 secretion (right) ( n = 3). b , d , e Data are from distinct healthy donors. Horizontal lines indicate the mean ± SEM. NS= non-significant; p -values were determined by one-way ANOVA with Tukey’s post hoc test ( b , d , g ).

    Article Snippet: For IL-12p70 and CD40L blocking experiments, anti-Human Il-12p70 (R&D Systems, cat #MAB219-100, 10 µg/ml final), anti-CD40 (BioLegend, cat #313019, clone HB14, 10 µg/ml final) or isotype control mouse IgG1 (BioLegend, cat #400166, clone MOPC21, 10 µg/ml final) were added in the corresponding wells.

    Techniques: Expressing, Control, Inhibition

    a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail of IL-12, IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .

    Journal: Nature Microbiology

    Article Title: Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function

    doi: 10.1038/s41564-024-01838-z

    Figure Lengend Snippet: a , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with media alone ((RPMI-1640/B27 with insulin; control) or a cocktail of IL-12, IL-1β, MCP-1 and IL-6 (‘cytokines’). Cytokine concentrations were either ‘Recovered cytokine mimic’ (IL-12, 0.007 fg ml −1 ; IL-1β, 0.01 fg ml −1 ; MCP-1, 0.01 fg ml −1 ; IL-6, 0.004 fg ml −1 ) or ‘PASC-CVS cytokine mimic’ (IL-12, 41 fg ml −1 ; IL-1β, 21 fg ml −1 ; MCP-1, 14 fg ml −1 ; IL-6, 21 fg ml −1 ) to reflect levels detected in the PASC-CVS cohorts. Data are pooled from two independent experiments. Graphs show mean ± s.e.m. Normal distribution of data was assessed with the Shapiro–Wilk test. Statistical significance was determined with a Kruskal–Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. b , Primary human cardiomyocytes were incubated for 24 h (top row) and 48 h (bottom row) with PASC-CVS or Recovered plasma. Data are displayed relative to cardiomyocytes treated with media alone. Mean ± s.e.m. is shown in all graphs. Statistical significance was determined with an ANCOVA adjusted for age, sex and/or site as covariates. Covariates were included in the analysis if statistically significant difference in the covariate was recorded between groups. Each donor is indicated by a unique symbol that is used consistently throughout all figures. Grey horizontal lines indicate the mean value derived from n = 22 Healthy donors. A description of the Healthy donor cohort is presented in Supplementary Table .

    Article Snippet: Surface-enhanced Raman scattering nanotags were conjugated with antibodies against IL-1β (MAB601, clone 2805), IL-6 (AF-206, polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910) or MCP-1 (MAB679, clone 23007) obtained from R&D Systems.

    Techniques: Incubation, Control, Comparison, Derivative Assay

    Human cardiomyocytes were incubated for 24 h (top line) or 48 h (bottom line) with media alone ((RPMI-1640/B27 with insulin; control) or individual pro-inflammatory cytokines. Cytokine concentrations were ‘PASC-CVS cytokine mimic’ (IL-12: 41fg/mL; IL-1β 21fg/mL; MCP-1 14fg/mL or IL-6 21fg/mL). Graphs show mean ± SEM. Normal distribution of data was assessed with the Shapiro-Wilk test. Mean ± SEM is shown. Statistical significance was determined with a Kruskal-Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. Data is pooled from three independent experiments.

    Journal: Nature Microbiology

    Article Title: Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function

    doi: 10.1038/s41564-024-01838-z

    Figure Lengend Snippet: Human cardiomyocytes were incubated for 24 h (top line) or 48 h (bottom line) with media alone ((RPMI-1640/B27 with insulin; control) or individual pro-inflammatory cytokines. Cytokine concentrations were ‘PASC-CVS cytokine mimic’ (IL-12: 41fg/mL; IL-1β 21fg/mL; MCP-1 14fg/mL or IL-6 21fg/mL). Graphs show mean ± SEM. Normal distribution of data was assessed with the Shapiro-Wilk test. Mean ± SEM is shown. Statistical significance was determined with a Kruskal-Wallis test with Dunn’s multiple comparison test or Welch ANOVA test and Dunnett’s multiple comparison test. Data is pooled from three independent experiments.

    Article Snippet: Surface-enhanced Raman scattering nanotags were conjugated with antibodies against IL-1β (MAB601, clone 2805), IL-6 (AF-206, polyclonal), IL-12p70 (MAB611, clone 24945), IL-12p70 (MAB219, clone 24910) or MCP-1 (MAB679, clone 23007) obtained from R&D Systems.

    Techniques: Incubation, Control, Comparison